Laser Scanning Confocal Microscope Maximum Magnification

The confocal laser scanning microscope clsm is a microscope which focuses only on a single focal plane and the unfocused plane will not be visualized.

Laser scanning confocal microscope maximum magnification.

Another drawback is that duv microscopes do not have the capability for surface topography 3d imaging. What is the maximum magnification of the scanning electron microscope. In the past the traditional laser microscope excited the whole thickness of the sample resulting in saturated blurry images and sometimes visualizing false colocalization images. The laser scanning microscope uses a scanning design called beam scanning where the laser image path is scanned in a raster pattern on the surface of the sample.

The confocal laser scanning microscope s aim was not to further increase magnification but to make clearer. Relatively thick specimens can be imaged in successive volumes by acquiring a series of sections along the optical z axis of the microscope. Capturing multiple two dimensional images at different depths in a sample enables the. This resulted in a costly microscope system with magnification limited to 1000x.

The scanning electron microscope has a magnification range from 15x to 200 000x reached in 25 steps and a resolution of 5. The laser scans across the object and an image is built up pixel by pixel on a screen.