Laser Scanning Confocal Microscope Magnification

The confocal laser scanning microscope s aim was not to further increase magnification but to make clearer.

Laser scanning confocal microscope magnification.

With confocal laser scanning microscopy clsm we can find out even more. Relatively thick specimens can be imaged in successive volumes by acquiring a series of sections along the optical z axis of the microscope. Fluorescent microscopy not only makes our images look good it also allows us to gain a better understanding of cells structures and tissue. Confocal microscopy most frequently confocal laser scanning microscopy clsm or laser confocal scanning microscopy lcsm is an optical imaging technique for increasing optical resolution and contrast of a micrograph by means of using a spatial pinhole to block out of focus light in image formation.

Clsm combines high resolution optical imaging with depth selectivity which allows us to do optical sectioning. Capturing multiple two dimensional images at different depths in a sample enables the. The laser scans across the object and an image is built up pixel by pixel on a screen. A confocal laser scanning microscope or also known as laser scanning confocal microscope is used to obtain high resolution images and generate 3d reconstructions through direct optical sectioning to provide clear images from a range of depths of thicker specimens for nanometer level imaging and measurement.

Laser scanning confocal microscopy laser scanning confocal microscopes employ a pair of pinhole apertures to limit the specimen focal plane to a confined volume approximately a micron in size.